Kinetic Spectrophotometric Methods for the Determination of Chloramphenicol in Pharmaceutical Preparations

Authors

  • Hind S Al-Ward Department of Chemistry, College of Sciences, University of Baghdad, Baghdad-Iraq.

Keywords:

chloramphenicol, kinetic spectrophotometry, oxidative–coupling reaction

Abstract

Simple and sensitive kinetic methods are described for the determination of chloramphenicol in pure form and pharmaceutical preparations. The methods are based on oxidative – coupling reaction between reduced chloramphenicol (by zinc powder and concentrated hydrochloric acid) with promethazine hydrochloride in the presence of sodium periodate yielding a highly colored product at room temperature, the reaction is followed spectrophotometriclly at λmax= 590 nm. Initial rate and fixed time (at 20 minutes) methods are utilized for concentration determination. The calibration graphs were linear in the concentration ranges (2-20 µg.ml-1) and (0.5-30 µg.ml-1) respectively. The results were validated statistically and checked through recovery studies, and have been applied successfully for the determination of chloramphenicol in commercial dosage forms  

Published

2018-07-05

Issue

Section

Articles

How to Cite

(1)
Kinetic Spectrophotometric Methods for the Determination of Chloramphenicol in Pharmaceutical Preparations. ANJS 2018, 15 (4), 22-30.