PCR Ribotyping for Determining the Diversity of Some Clinical Pseudomonas Aeruginosa Isolates

Authors

  • Khulod Ibraheem Hassan Department of Food Science, Faculty of Agricultural Science, Sulamani University.
  • Saman Rafeeq Abdullah Department of Biology, Koya Technical Institute.
  • Khidir Hawrami Koya University, University Dean.

Keywords:

Pseudomonas aeruginosa, genetic diversity, PCR, Ribotyping

Abstract

To study the genetic diversity between deferent strains of P. aeruginosa, this study was carried out between August and December 2010 by collecting 165 clinical samples from wounds, burn, ear and urinary tract infection taken from general hospitals of Sulaimani, Erbil and Koya governorates. After identifying P. aeruginos based upon culture methods using the selective media (cetrimide agar) coupled with biochemical tests, Polymerase chain reaction (PCR) method were used for identifying P. aeruginosa using primers targeting 16S rRNA. PCR analysis of 16S-23S internal transcribed spacer primer targeting the interspaced regions between the 16 and 23S rRNA genes (PCR ribotyping) was evaluated for it s effectiveness to differentiate between the isolates, the results show that the clinical isolates of P. aeruginosa belongs to three ribotypes, which were showed in one to three bands in each isolate of P. aeruginosa.

Published

2018-07-02

Issue

Section

Articles

How to Cite

(1)
PCR Ribotyping for Determining the Diversity of Some Clinical Pseudomonas Aeruginosa Isolates. ANJS 2018, 17 (2), 159-164.