Biochemical changes of alkaline phosphatase in tissue homogenate of the developing femurs in chick embryos after in ovo calcium carbonate nanoparticles inoculation
Keywords:
Chick eggs , Embryo , Calcium carbonate , Nanoparticles , Femur, Osteogenesis, Tissue homogenate, Alkaline phosphataseAbstract
This study oriented to provide evidences related to effects of calcium carbonate nanoparticles (CCN) on the osteogenesis of the developing femur in chick embryos. The 20 fertilized chick eggs used in this study divided into two a control and experimental groups. The control group of eggs injected with normal saline pre-incubation, while eggs of the experimental group injected with 500 g/mL hydrocolloid of CCN in 0.2 mL volume per egg.
The homogenate of the femur obtained from the control and experimental groups show statistically significant variations when comparing the level of alkaline phosphatase in the tissue of the control group at day 13 with that of the experimental group at day 13 and day 14. The alkaline phosphatase level as a marker of bone metabolism in the femur tissue homogenate of the experimental group revealed highly significant increased concentration compared to that of the control group at both day 13 and 14. This characteristic finding is associated with the effect of in-ovo inoculation of CCN on the femur tissue homogenate in pre-hatching chick embryos during osteoid formation and mineralization.
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