Comparative Study of Total Phenolics and Antioxidants in ASTRAGLUS STOCKSII by using Methanol, Ethanol and Acetone and to Evaluate its Anti-Inflammatory Activity

  • Iqra Arshad Institute of Biochemistry, University of Balochistan, Quetta, 87300- Pakistan
  • Muhammad Ayub Kakar Institute of Biochemistry, University of Balochistan, Quetta, 87300- Pakistan
  • Attiq- ur-Rehman Kakar Department of Chemistry, University of Balochistan, Quetta, 87300- Pakistan
  • Noor Hassan Kakar Institute of Biochemistry, University of Balochistan, Quetta, 87300- Pakistan
  • Saeeda Khanam Institute of Biochemistry, University of Balochistan, Quetta, 87300- Pakistan
  • Rehana Kamal Department of Obstetrics and Gynecology, Bolan University of Medical and Health Sciences, Quetta 87300, Pakistan
  • Atta Muhammad Government Boys Degree College Khanozai-86600, Education Department Balochistan, Pakistan
Keywords: Astragalus Stocksii, Antioxidants, Anti-inflammatory, DPPH method, TAC method

Abstract

Astragalus Stocksii a local flowering plant found in Hana Valley, Quetta Pakistan, used as home medicinal plant form many years. It is an encouraging source of bioactive compounds and has been conventionally manipulated for the coverage of several diseases. The present study based on the evaluation of phenolics, antioxidants and anti-inflammatory activities of Astragalus Stocksii by using three different solvents, i.e.; acetone, ethanol and methanol. The results showed that extraction yield depends upon the type of the solvents used for extraction. Among these, extraction in methanol has the highest phenolic contents (10.086±0.08mg CE/g DW), total antioxidant capacity (TAC, IC50 =14.50±0.01 µg/mL, phospho-molibdenum method, IC50 = 66.62± 0.03 µg/mL by DPPH method) and high anti-inflammatory activity (IC50 =75.0 ±2.5 µg/mL, oxidative burst assay). These results indicate that methanol is the best solvent for extraction of phenolic and antioxidants in Astragalus Stocksii. The high results of anti-inflammatoryfor Astragalus Stocksii suggests its use in pharmaceutical and nutraceutical industries.

Published
2021-09-30
How to Cite
Arshad, I., Ayub Kakar, M., ur-Rehman Kakar, A.-, Hassan Kakar, N., Khanam, S., Kamal, R., & Muhammad, A. (2021). Comparative Study of Total Phenolics and Antioxidants in ASTRAGLUS STOCKSII by using Methanol, Ethanol and Acetone and to Evaluate its Anti-Inflammatory Activity. Al-Nahrain Journal of Science, 24(3), 16-22. Retrieved from https://anjs.edu.iq/index.php/anjs/article/view/2421
Section
Articles