Determination of Sulfamethoxazole in Pure and Pharmaceutical Samples by Using Direct Method for Calibration Curve of Normal Spectrum of UV-Spectrophotometry

  • Sarra A. Abrahem Dept. of Chemistry, College of Science at Al-Nahrain University
  • Khaleda H. Al-Saidi
Keywords: UV-spectrophotometry, Normal spectrum, Sulfamethoxazole, Analytical methods, Direct method

Abstract

Normal spectra for sulfamethoxazole solutions were developed and used for the determination of sulfamethoxazole (SMX) antibiotic by using zero-crossing technique and simultaneously determining (SMX) at wavelength 259.00 nm. The correlation coefficient of the calibration curve for the normal spectrum was 0.9990. Linearity was maintained by using concentrations (0.990´10-4 M, 0.996´10-4 M, 0.999´10-4 M, 1.004´10-4 M, 1.005´10-4 M) and the percentage recovery of sulfamethoxazole samples were (99.00%, 99.60%, 99.90 %, 100.40 % and 100.50 %). A statistical analysis confirmed, a precision with accuracy for simultaneous fixing of (SMX). In addition, a British pharmacopoeia method was compared with the method used in this paper using F test.

Published
2020-11-30
How to Cite
A. Abrahem, S., & H. Al-Saidi, K. (2020). Determination of Sulfamethoxazole in Pure and Pharmaceutical Samples by Using Direct Method for Calibration Curve of Normal Spectrum of UV-Spectrophotometry. Al-Nahrain Journal of Science, 23(4), 1-4. Retrieved from https://anjs.edu.iq/index.php/anjs/article/view/2344
Section
Articles