Three Simple Spectrophotmetric Methods Using for Estimation of Vancomycin in Pure and Vial Injection

  • Ala'a Abdullwahid Jasim Department of Chemistry, College of Science-University of AL-Mustansiriyah- Baghdad-Iraq
Keywords: Vancomycin, Zero-order, Area under curve, First derivative method, Spectrophotometric


Three rapid, accurate and ecological friendly spectrophotometric methods are used for determination of  Vancomycin at wavelength 200 nm for the  zero-order method, 195-205 nm for the area under the curve (AUC) method and 200 nm for  the first derivative method(D1). A calibration graph shows that  Beer's law is obeyed over the concentration range between  4-16 mg.L-1 for all three methods with a correlation coefficient of 0.994, 0.998 and  0.997 for zero-order, AUC and  D1 at valley 200 nm, respectively. Average of  Rec.% were found to be of 100.870, 101.425 and  99.515 for zero-order, AUC and  D1 at valley 200 nm, respectively. Statistical analysis of data reveals that  all three methods are precise, accurate and suitable for the application Vancomycin in pure and pharmaceutical preparations.


[1] Murray, B. E.; Arias, C. A.; Nannini, E. C.; "Glycopeptides (vancomycin and teicoplanin), streptogramins (quinupristin-dalfopristin), lipopeptides (daptomycin), and lipoglycopeptides (telavancin) Mandell, Douglas, and Bennett’s principles and practice of infectious diseases", 8th edition Philadelphia, Saunders; 377-400; 2015.
[2] Hu, Q.; Peng, H.; Rao, X. "Molecular events for promotion of vancomycin resistance in vancomycin intermediate Staphylococcus Aureus", Frontiers in Microbiology 7, 1601, 2011.
[3] Al-Abachi, M.; Hadi, H.; Yousef, F.; "flow injection spectrophotometric determination of vancomycin hydrochloride in pharmaceutical preparation using diazotized metoclopramide", Al-Nahrain J. Sci. 18(1), 9-19, 2015.
[4] Abed, S. S.; Hussein, O. T.; "Spectrophotometric determination of vancomycin hydrochloride (batch and flow injection) using o-nitroaniline as diazotized chrmogenic reagent", Iraqi. J. Sci. 56, 3025-3035, 2015.
[5] Belal, F.; El-Ashry, S. M.; El-kerdawy, M. M.; El-wassef, D.R. "Voltametric determination of vancomycin in dosage forms through treatment with nitrous acid", Arzneimi Helforschung 51(9), 763-768, 2001.
[6] Hu, L.; Ling-Yinm C.; Peng, Z.; "Simultaneous and direct determination of vanvomycin and cephalexin in human plasma by using HPLC-DAD coupled with second-order calibration Algorithms"; J. Ana. Chem. 2012, 1-8, 2012.
[7] Hagihara, M.; Sutherland, C.; Nicolau, D. P.; "Developed of HPLC methods for the determination of vancomycine in human plasma, mouse serum and bronchoalveolar lavage fluid"; J. Chrom. Sci. 2012, 1-7, 2012.
[8] Gurnpaday, B. M.; Disha, N. S.; "Stability indicating HPLC method for the simultaneous determination of ceftriaxone and vancomycin in pharmaceutical formulation"; J. Chrom. Tech. 207(4), 1-5, 2013.
[9] Sánchez‐Hernández, L.; Domínguez‐Vega, E.; Montealegre, C.; Castro‐Puyana, M.; Marina, M. L.; Crego, A. L. "Potential of vancomycin for the enantiomeric resolution of FMOC‐amino acids by capillary electrophoresis‐ion‐trap‐mass spectrometry"; Electrophoresis 35(9), 1244-1250, 2014.
[10] El-didamony, A.K.; Amin, A. S.; Ghoneim, A. K.; Telebany, A. M.; "Indirect spectrophotometric determination of gentamicin and vancomycine antibiotics based on their oxidation by potassium permanganate"; Central Euo. J. Cem. 4(4), 708-722, 2006.
[11] Prashanth, K. N.; Basavaiah, K.; Xavier, C. M.; "Development and validation of UV-spectrophotometric methods for the determination of sumatriptan succinate in bulk and pharmaceutical dosage form and its degradation behavior under varied stress conditions"; J. Assoc. Arab Univ. Basic Appl. Sci. 15, 34-52, 2014.
[12] El-Din, M. K.; Ibrahim, F.; El-Deen, A. K.; Shimizu, K.; "Stability-indicating spectrofluorimetric method with enhanced sensitivity for determination of vancomycin hydrochloride in pharmaceuticals and spiked human plasma: Application to degradation kinetics"; J. Food Drug Anal. 26(2), 834-841, 2018.
[13] Abdullah, N. S.; Hassan, M. A; Hassan, R. O.; "Spectrophotometric determination of chlorthalidone in pharmaceutical formulations using different order derivative methods". Arab. J. Chem. 10, S3426-S3433, 2014.
[14] Khandare, B.; Musle, A. C.; Arole, S. S.; Popalghat, P. V.; "Spectrophotometric Determination of Olmutinib in Bulk by Area under Curve and First Order Derivative Methods and its Validation as per ICH guidelines"; J. Drug Del. Therapeutics 9(4-A), 349-354, 2019.
[15] Nassar, M.; Attia, K.; Mohamad, A.; Said, R.; Abdel-Monem, A. "Quantitative Estimation and Validation of Rafoxanide in Presence of its Alkali-Induced Degradation Product by Different Spectrophotometric Methods"; J. Adv. Pharm. Res. 2(1), 20-27, 2018.
[16] Shetty, P. R.; Patil, D. D.; "Applications of simultaneous equation method and derivative method for the determination of rabeprazole sodium and levosulpiride in pharmaceutical dosage form and dissolution samples"; J. Assoc. Arab Univ. Basic Appl. Sci. 15, 53–60, 2014.
[17] Al-Beea, R. F.; Abbas, H. A.; Taqi, R. M.; Abdulabbas, D. A.; Abed, M. A.; "Estimation of the Folic Acid Using Zero Order, Area Under Curve and First Derivative Spectrophotometric Methods in Pure and Marketed Tablet Formulations", Al-Nahrain J. Sci. 20 (3), 34-41, 2017.
[18] Dhobale, S.; Narad, V.; Gaikwad, D.; "Estimation of Lovastatin in Pharmaceutical Formulation by Area under Curve Spectrophotometric Method", Iraqi Int. J. Appl. Sci. 6(3), 85-93, 2017.
How to Cite
Abdullwahid Jasim, A. (2020). Three Simple Spectrophotmetric Methods Using for Estimation of Vancomycin in Pure and Vial Injection. Al-Nahrain Journal of Science, 23(1), 35-42. Retrieved from