Molecular Study of 16SrRNA Gene in Enterobacteriaceae isolated from Iraqi Patients

Authors

  • Shahad Hisham Mahmood Department of Biology, College of Sciences, University of Anbar, Anbar-Iraq.
  • Ahmed Mohamed Turkey Department of Biology, College of Sciences, University of Anbar, Anbar-Iraq.
  • Ilham Abdul Hadi Khalaf Al-Razi Center for Research and Diagnostic Kits, Ministry of Industry and Minerals, Baghdad-Iraq.

Keywords:

Enterobacteriaceae, 16S rRNA, PCR, Molecular Identification

Abstract

This study included the collection of 204 clinical and non-clinical samples from Ibn-Al-Baladi childbirth Hospital, Al-Yarmouk Teaching Hospital and Imam Ali Hospital in Baghdad, from both genders of different ages. The collected samples were distributed according to the collection source (urine, wounds, burns, feces, Tigris River water in Baghdad and soil samples). A total of  isolates of Escherichia coli (48.43 %), 15 isolates Klebsiella pneumoniae (23.43 %), 10 isolates of Enterobacter cloacae (15.62 %) and 8 isolates of proteus mirabilis (12.50 %) were isolated and identified based on microscopic culture, conventional methods, VITEK 2 and molecular identification of 16 SrRNA gene. Our investigations indicated that Escherichia coli and Klebsiella pneumoniae species represent the most frequent isolates, whereas both Enterobacter cloacae and Proteus mirabilis were the less frequent species. The results of the analysis of the sequencing of selected 21 isolates were deposited in the National Center for Biotechnology Information (NCBI) belong to the four species (From LC314477.1 to LC314497.1).

Published

2018-10-25

Issue

Section

Articles

How to Cite

(1)
Molecular Study of 16SrRNA Gene in Enterobacteriaceae Isolated from Iraqi Patients. ANJS 2018, No. 1, 78-87.