In Vitro Evaluation of Nanochitosan Derivatives and Streptomycin on RD Cell Line and Leishmania Tropica

  • Ali Shehab Ahmed College of Biotechnology, University of Al- Nahrian, Baghdad-Iraq.
  • Muna Mohammed Khayri
  • Mohammed M. F Al-Halbosiy Biotechnology Research Center, University of Al-Nahrian, Baghdad-Iraq.
Keywords: Carboxymethylnanochitosan, Encapsulation, RD cell line, Leishmania tropica, Growth Inhibition%

Abstract

The study aimed to evaluate in vitro the efficiency of the nano-encapsulated streptomycin within carboxymethylnanochitosan synthesized by an acid-base method on Rhabdomyosarcoma RD cell line and Leishmania tropica. Results showed that the concentrations 100 and 50 µg/ml of encapsulated streptomycin were more effective than 50 and 100 µg/ml of streptomycin alone and inhibited the growth of RD cells to 56 and 35 vs. 19.45 and 23% respectively after 24 hrs of incubation. Carboxymethylnanochitosan at a concentration of 50 µg/ml has the anticancer ability since it inhibited the growth of RD cells up to 20% in comparison with 19% for streptomycin at the same concentration. The growth inhibition of L. tropica in the presence of streptomycin, carboxymethylnanochitosan and streptomycin encapsulated within carboxymethylnanochitosan at 100 µg/ml for each was 34.4, 36 and 29.5% respectively. Carboxymethylnanochitosan is then considered the most effective inhibitor of L. tropica growth.
Published
2017-12-01
How to Cite
Ahmed, A. S., Khayri, M. M., & Al-Halbosiy, M. M. F. (2017). In Vitro Evaluation of Nanochitosan Derivatives and Streptomycin on RD Cell Line and Leishmania Tropica. Al-Nahrain Journal of Science, 20(4), 77-80. Retrieved from https://anjs.edu.iq/index.php/anjs/article/view/129
Section
Articles