Anticancer Studies of Extracts and Fractions of Hertia Intermedia
Keywords:
Hertia Intermedia, Methanolic extract, 3T3, MCF-7, Hela, Cell linesAbstract
This study was conducted to prepare the extract from medicinal plant Hertia intermedia and its evaluation for presence of anticancer activity. The medicinal plant was collected and shade dried then crushed to convert it into powdered form. The powdered form of the plant was soaked for 15 days in an organic solvent methanol (CH3OH). The extract obtained passed through the filtration process followed by vaporization of the residual solvent using rotary evaporator. The methanol extract of Hertia Intermedia was used to prepare different fractions of solvent using aqueous dichloromethane, benzene, butanol, chloroform, ethyl acetate and n-hexane. Methanol extract, aqueous extract, butanol extract and extract of Hertia intermedia did not show any activity against cancerous cell lines. Hertia intermedia did not show any noticeable activity and remains inactive ROS against HeLa cell line, 3T3 cell line, MCF-7 cell line (cancerous cell). Hertia intermedia did not contain any bioactivity against cancerous or reactive oxygen species which is the initiator or producing cancer.
References
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[3] Desai, A. G.; Qazi, G. N.; Ganju, R. K.; El-Tamer, M.; Singh, J.; Saxena, A. K.; Bhat, H. K.; “Medicinal plants and cancer chemoprevention”; Curr. Drug metabolism 9, 581-591, 2008.
[4] Singh, S. S.; Pandey, S. C.; Srivastava, S.; Gupta, S.; Patro, B.; Ghosh, A. C.; “Tinospora Cordifolia: Chemistry and Medicinal Properties”; Ind. J. Pharm 35, 83-91, 2003.
[5] Gordaliza, M.; “Natural products as leads to anticancer drugs”; Clinical and Translational Oncology 9, 767-776, 2007.
[6] Al-Janabi, N. M.; Al-Halbosiy, M. M.; Al-Badri, S. R.; “The Inhibitory Action of Capparisspinosa Leaves sagainst the Tumor and Leishmania”; Al-Nahrain J. Sci. 0(2), 53-58, 2019.
[7] Allawi, M. H.; Abed, M. Q.; “A Study of Anticancer Activity for Partial Purified Urease Isolated from Lagonychium farctum Plant”; Al-Nahrain J. Sci. 0(1), 95-99, 2018.
[8] Rashmi, R.; Kumar, S.; Karunagaran, D.; “Ectopic expression of Hsp70 confers resistance and silencing its expression sensitizes human colon cancer cells to curcumin – induced apoptosis”; Carcinogenesis 25, 179-187, 2004.
[9] Ikezaki, S.; Nishikawa, A.; Furukawa, F.; Kudo, K.; Nakamura, H.; Tamura, K.; Mori, H.; “Chemopreventive effects of curcumin on glandular stomach carcinogenesis induced by N-Methyl-N'-nitro-N-nitrosoguanidine and sodium chloride in rats”; Anticancer Research 21, 3407-3411, 2001.
[10] Damjanović, A.; Zdunić, G.; Šavikin, K.; Mandić, B.; Jadranin, M.; Matić, I. Z.; Stanojković, T.; “Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis”; Bangladesh J. Pharma. 11, 741-749, 2016.
[11] Sultana, S.; Asif, H. M.; Nazar, H. M.; Akhtar, N.; Rehman, J. U.; Rehman, R. U.; “Medicinal plants combating against cancer-a green anticancer approach” Asian Pac. J. Cancer Prev. 15, 4385-94, 2014.
[12] Akhgar, M. R.; Akhgar, D.; Ghazanfari, M.; Shariatifar; “Chemical composition and antibacterial activity of the leaf essential oil from Hertia intermedia”; Chem. Nat. Compd. 48, 2, 2015.
[13] Hashemi, A.; Abediankenari, S.; “Suppressive effect of Fig (Ficus carica) latexon esophageal cancer cell proliferation”; Acta Facultatis Med. Naissensis 30, 93, 2013.
[14] Jahangir, K. A.; Muhammad, A. P.; Abdul, M. K.; Ajab, K. T.; Nazima, Y. K.; Javed, K.; Asmatullah, K.; Samiullah, G.; “Analysis and Antileishmanial activity of dichloromethane fraction of Allium cepa (DFAC) in Vitro”; Internat. J. Pharma. Sci. Bio. 7, 40-5, 2016.
[15] Sultana, S.; Asif, H. M.; Nazar, H. M.; Akhtar, N.; Rehman, J. U.; Rehman, R. U.; “Medicinal plants combating against cancer-a green anticancer approach”; Asian Pac. J. Cancer. Prev. 15, 4385-94, 2014.
[16] Sheeja, L.; Lakshmi, D.; Bharadwaj, S.; Parveen, K. S.; “Anticancer activity of phytol purified from Gracilaria edulis against human breast cancer cell line (MCF-7)”; Int. J. Curr. Sci. 19, 36-46, 2016.
[17] Hashemi, S. A.; Abediankenari, S.; “Suppressive effect of fig (Ficus carica) latex on esophageal cancer cell proliferation”; Acta Facultatis Medicae Naissensis 30, 93-96, 2013.
[18] Ma, X.; Wang, Z.; “Anticancer drug discovery in the future: an evolutionary perspective”; Drug Discovery Today 14, 1136-1142, 2009.
[19] Zeng, Y. W.; Liu, X. Z.; Lv, Z. C.; Peng, Y. H.; “Effects of Ficus hirta Vahl. (Wuzhimaotao) extracts on growth inhibition of HeLa cells”; Experimental and Toxicologic Pathology 64, 743-749, 2012.
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Published
2020-03-04
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[1]
“Anticancer Studies of Extracts and Fractions of Hertia Intermedia”, ANJS, vol. 23, no. 1, pp. 43–46, Mar. 2020, Accessed: Apr. 25, 2024. [Online]. Available: https://anjs.edu.iq/index.php/anjs/article/view/2239
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